Juventus:
A win for the Italian giants would secure their place in the last 16, but a loss could leave their hopes hanging in the balance.
Gianluigi Buffon was left out of the defeat to Sampdoria on the weekend but is set to be recalled by Massimiliano Allegri for Wednesday's crucial tie.
Andrea Barzagli, Alex Sandro and Douglas Costa will also be pushing for a start.
Barcelona:
Ernesto Valverde's men can clinch top spot in Group D if they avoid defeat in this encounter.
Gerard Pique, who is suspended for Barcelona's top of the table La Liga clash against Valencia on Sunday, is expected to start, but Ousmane Dembele, Arda Turan, Javier Mascherano, Andre Gomes, Sergi Roberto, and Rafinha all remain sidelined.
Recent form:
Possible lineups:
Juventus: Buffon, Lichtsteiner, Barzagli, Chiellini, Sandro, Pjanic, Matuidi, Costa, Dybala, Mandzukic, Higuain
Barcelona: Ter Stegen, Vidal, Pique, Umtiti, Alba, Iniesta, Paulinho, Busquets, Deulofeu, Suarez, Messi